Proteinase Activity and Receptor Cleavage

Proteinase activity and receptor cleavage: mechanism for insulin resistance in the spontaneously hypertensive rat.

Arterial hypertension is associated with organ dysfunctions, but the mechanisms are uncertain. We hypothesized that enhanced proteolytic activity in the microcirculation of spontaneously hypertensive rats (SHRs) may be a pathophysiological mechanism causing cell membrane receptor cleavage and examine this for 2 different receptors. Immunohistochemistry of matrix-degrading metalloproteinases (ma...

Activation Activity and Proteinase-Activated Receptor-2 Allergens Is Dependent on Proteinase Mucosal Allergic Sensitization to Cockroach

Potyviral NIa proteinase, a proteinase with novel deoxyribonuclease activity.

The NIa proteinase from pepper vein banding virus (PVBV) is a sequence-specific proteinase required for processing of viral polyprotein in the cytoplasm. It accumulates in the nucleus of the infected plant cell and forms inclusion bodies. The function of this protein in the nucleus is not clear. The purified recombinant NIa proteinase was active, and the mutation of the catalytic residues His-4...

Primary structure of bovine endothelin ETB receptor and identification of signal peptidase and metal proteinase cleavage sites.

A cDNA clone corresponding to the entire coding region of the bovine ETB endothelin receptor mRNA was isolated from a lung cDNA library and sequenced. The cDNA encodes 441 amino acids: 26 constituting an NH2-terminal signal peptide and 415 constituting the mature receptor. The signal peptidase cleavage site was determined by direct amino acid sequencing of purified receptor. A comparison of the...

Glycosylation and Proteinase-Activated Receptor Function

N-linked glycosylation is a post-translational modification that enables the cell to regulate protein function without recourse to the genome. The Proteinase-Activated Receptors (PAR) each possesses a number of N-linked glycosylation sites on their extracellular domains. However, little is currently known about the relative glycosylation status of each family member and about the influence that...